보고서 정보
주관연구기관 |
국립원예특작과학원 National Institute of Horticultural and Herbal Science |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2014-02 |
과제시작연도 |
2011 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201400010975 |
과제고유번호 |
1395021534 |
사업명 |
농업현장실용화기술개발 |
DB 구축일자 |
2014-07-05
|
DOI |
https://doi.org/10.23000/TRKO201400010975 |
초록
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Ⅳ. 연구개발결과
1. 새송이 고품질 다수성 품종 개발
○ 육종효율 증진 및 품종 구분을 위해 미토콘드리아 microsatellite를 이용하여 느타리버섯류 품종구분 뿐만아니라 육종효율 증진을 위한 마커로서 활용하였다
○ 큰느타리는 자실체 발생량이 많아 솎기작업으로 발생개체수를 줄이고 있어, 솎음작업에 소요되는 노동력 절감을 위해 환경조절에 의한 발생개체수가 적으면서 고품질 다수성 품종 ‘송아’를 육성하였다.
○ 느타리를 재배하다가 전업한 큰느타리 농가에서는 수분을 높게하여 재배하고 있는 실정에 맞게 내습성이
Ⅳ. 연구개발결과
1. 새송이 고품질 다수성 품종 개발
○ 육종효율 증진 및 품종 구분을 위해 미토콘드리아 microsatellite를 이용하여 느타리버섯류 품종구분 뿐만아니라 육종효율 증진을 위한 마커로서 활용하였다
○ 큰느타리는 자실체 발생량이 많아 솎기작업으로 발생개체수를 줄이고 있어, 솎음작업에 소요되는 노동력 절감을 위해 환경조절에 의한 발생개체수가 적으면서 고품질 다수성 품종 ‘송아’를 육성하였다.
○ 느타리를 재배하다가 전업한 큰느타리 농가에서는 수분을 높게하여 재배하고 있는 실정에 맞게 내습성이 강한 ‘설송’을 육성하였다.
2. 새송이의 육종모본 수집 및 특성 평가
○ 국내외 큰느타리 및 큰느타리 변종 유전자원 228균주를 수집하여 균사체 배양특성과 지역별 수집균주의 특성을 평가하였다.
○ PCR을 통해 큰느타리와 유연관계인 느타리 종, 속간 유연관계 분석을 통하여 큰느타리 버섯의 유연관계를 확립하였고, 특성평가 및 유연관계가 확립된 균주는 육종가에게 분양하여 품종을 육성하게 하였다.
○ 육성된 품종의 우리고유 품종으로 인증하기 위해 육성된 품종을 수집하여 PCR을 이용한 분자마커를 개발하여 활용하였다.
3. 새송이 소발생형 품종 개발
○ 큰느타리버섯 중에서 가장 많이 생산되고 있는 큰느타리2호의 대체품종 육성을 통해 ‘곤지3호’를 육성하였다.
○ 솎음작업에 소요되는 노동력 절감을 위해 소발생형 ‘새곤지’ 품종을 육성하여 농가실증 시험을 하고 있다.
4. 새송이 최적배지 및 사료화 기술개발
○ 버섯 및 축산농가의 경영비 절감을 위한 대체자원으로써 농산부산물이나 산업부산물을 버섯배지로 활용하기 위해 화학적 성분분석을 통해 대체 가능성을 타진하였다.
○ 수확후배지의 재활용 위한 방법으로 사료화를 시도하여 혐기적 발효방법으로 수확후배지의 30%를 첨가한 배지에서 효율성을 보여 농가에 보급함으로서 실효성이 있음을 확인하였다.
Abstract
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I 새송이 고품질 다수성 품종 개발
King oyster mushroom, P leurotus eryngii among P leurotus sp., are mostly produced in Korea and exported to other countries. Most P leurotus eryngii strains grown in Korea were originated from foreign countries, which the royalty problems in these days. To overcome this proble
I 새송이 고품질 다수성 품종 개발
King oyster mushroom, P leurotus eryngii among P leurotus sp., are mostly produced in Korea and exported to other countries. Most P leurotus eryngii strains grown in Korea were originated from foreign countries, which the royalty problems in these days. To overcome this problem, we need to develop new Korean strains and it molecular marker from P . ostreatus, P . eryngii var. ferulae and P . eryngii var. nebrodensis etc. We developed the primer using mitochondrial microsatellite gene. PCR amplification using these primers indicated that microsatellite primer was useful for P leurotus variety discriminations
as a molecular marker. And also the two mating factors with their mating type loci are used as marker for breeding. It improves the breeding program when the breeder is able to quickly compatible strains in a given set of progeny. Mating type genes within progeny may be more easily identified by the use of direct PCR. We analyzed homeodoain(HD1 and HD2) and pheromone receptor genes as molecular markers for breeding using mating type A and B of P leurotus sp. by direct PCR. To develop a new cultivar of King oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of
Di-mon crossing between monokaryotic strains derived from ASI 2824(Keunneutari No.2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-51(G09-21-10 x 2844-11) was shown the best cultural characteristics, selected to be a new cultivar and named as ‘Song-A’.
This new cultivar ‘Song-A’ of P leurotus eryngii is characterized by a small number of primordia formation and the stip is thick and long. Therefore, we expect that this new strain will save of labor and cost by without culling work. The Pe21-53(G09-21-10 x ASI 2844-9) was shown the best cultural characteristics, selected to be a new cultivar and designated as ' Seolsong' . The ' Seolsong' was distinctly formed incompatibility line in the confrontation growth of parental strains Keunneutari No. 2, Aeryni 3 and ASI 2844.
Particularly, it was tolerant of high moisture above 90% during the growth period after primodia formation.
II 새송이의 육종모본 수집 및 특성 평가
To prepare the good parental lines for breeding of high quality King oyster varities, we collected 228 strains of P . eryngii and its related species of P . ferulae and P . nebredonsis.
This study was carried out to determine the most suitable medium and culture conditions such as temperature, pH, carbon and nitrogen sources, and solid media for good mycelium growth. The best temperature and pH values for the mycelial growth were 25°C∼30℃ and 6.0∼9.0, respectively. The carbon sources having significantly favorable effects on mycelium growth dextrose and glucose. Arginine and Ammonium acetate were the most suitable nitrogen sources. The best solid medium for mycelial growth was recorded in Hamada and mushroom complete media To develop the specic SCAR markers for Korean strains of P . eryngii, 50 strains of P . eryngii were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from RAPD polymorhic band specific to Korean strains of P . eryngii. The SCAR primer pair ‘INU-1’ was amplified from a 1,289bp fragment originated from IUM 5208 strain and ‘INU-6’ was amplified from a 639bp fragment originated from IUM 5198 strain. The Blast search for the sequences showed that no homology was found in any mushroom species. The results showed that the SCAR marker made from RAPD specific band of Korean strains tested can be clearly distinguished this strains from other P leurotus eryngii strains tested.
III 새송이 소발생형 품종 개발
We produce P leurotus eryngii mushroom about 50,605 ton per year and this mushroom is the third major mushroom in Korea. We are producing this mushroom by bottle culture system. The almost P leurotus eryngii mushroom farmers in Korea remove a lot of fruit body to harvest fruit body of big and thick stipe, remained one or two fruit bodies per bottle. Much of labor power is spent to remove the fruit body. For saving labor power spent to remove fruit body, we have bred new strains of P leurotus eryngii which made a few number of fruit body per bottle for five years.
We selected three mono-nuclear strains(E09-21, E09-58, E09-118) which made a few number of fruit body by mating GMPE25082 making a lot of fruit body and strains having good shape. After we made mating the three mono-nuclear strains selected at first year and strains having good fruit body shape, we selected 32 hybrid strains. Among them, 6 strains making a few number of fruit body as well as having good fruit body shape were selected and applied for productivity test.
After we made mating hybrid strains selected at second year, we selected 24 strains. 10 strains among them, we applied for productivity test. We did growing test at farms with 3 strains(E11-685, E12-176, E12-458) selected finally from productivity test. E12-176 strain made less number of fruit body than control strain and the yield of it was same as control strain. So we selected E12-176 strain as final strain making a few number of fruit body.
IV 새송이 최적배지 및 사료화 기술개발
Spent mushroom substrates(SMS) is a by-product remaining after a crop of mushrooms.
The mushroom substrates is are same feed ingredient as corncobs, rice brown, wheat brown, cotton seeds and beet pulp. During the mushroom cultivation, mushrooms was used 15-25% of mushroom substrates and 75-85% of mushroom ubstrates was remained in the SMS. The chemical composition of SMS was similar with commercial animal feed.
About 6 thermophilic strains were isolated from SMS (P leurotus eryngii). Among of them, one isolate, designated CS21, showed the antifungal activity against Aspergillus flavus producing mycotoxin and isolated fungus in this study on PDA medium, potentially.
The strain CS21 was produced cellulase, mannanase and xylanase as hydrolase. The strain CS21 was identified as members of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain CS21 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to B. subtilis with 16S rDNA gene sequence similarity of 99.9%.
On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain CS21 was classified within the genus Bacillus, for which the name Bacillus subtilis CS21 is proposed. For the mass production of B. subtilis CS21, the molasses (5%) and soybean meal (3%) was used as a carbon source and nitrogen source.
In this study we was carried out to investigate the feeding effects of dietary supplementation of fermented spent mushroom (P leurotus eryngii) substrates with B. subtilis CS21 and Saccharomyces sp. UJ14 (F-SMS) on growth performance and carcass
characteristics of Hanwoo steers. Twenty Hanwoo steers were allocated into two feeding groups and assigned equally to two dietary treatments; Control (commercial formula feed for Hanwoo steers) and TMR including 30% F-SMS.
The nutritional values of TMR including 30% F-SMS was higher crude protein and TDN was not significantly difference with commercial formula feed (p< 0.05). Feed intake was significantly greater in the TMR including 30% F-SMS than control (p< 0.05), but body weight gain and carcass grades were not influenced by the experimental diets.
Based on this study, fermented spent mushroom (P leurotus eryngii) substrate with Bacillus subtilis CS21 and Saccharomyces sp. UJ14 is able to use as an ingredient feed in TMR for Hanwoo steer.
목차 Contents
- 표지 ... 1
- 제 출 문 ... 2
- 요 약 문 ... 3
- SUMMARY ... 6
- 목차 ... 9
- 제1장 서 론 ... 10
- I 새송이 고품질 다수성 품종 개발 ... 10
- II 새송이의 육종모본 수집 및 특성 평가 ... 10
- III 새송이 소발생형 품종 개발 ... 12
- IV 새송이 최적배지 및 사료화 기술개발 ... 13
- 제2장 국내외 기술개발 현황 ... 15
- I 새송이 고품질 다수성 품종 개발 ... 15
- II 새송이의 육종모본 수집 및 특성 평가 ... 15
- III 새송이 소발생형 품종 개발 ... 15
- IV 새송이 최적배지 및 사료화 기술개발 ... 16
- 제3장 연구개발 수행 내용 및 결과 ... 18
- I 새송이 고품질 다수성 품종 개발 ... 18
- II 새송이의 육종모본 수집 및 특성 평가 ... 32
- III 새송이 고품질 다수성 품종 개발 ... 65
- IV 새송이 고품질 다수성 품종 개발 ... 80
- 제4장 연구개발목표 달성도 및 대외기여도 ... 109
- I 새송이 고품질 다수성 품종 개발 ... 109
- II 새송이의 육종모본 수집 및 특성 평가 ... 110
- III 새송이 소발생형 품종 개발 ... 111
- IV 새송이 최적배지 및 사료화 기술개발 ... 112
- 제5장 연구개발결과의 활용계획 ... 122
- 1절 : 추가 연구의 필요성 및 타 연구에의 응용 ... 122
- 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 124
- 제7장 기타 중요 변동사항 ... 125
- 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 126
- 제9장 참고문헌 ... 127
- 끝페이지 ... 135
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